This study was performed for the purpose of detecting the effect of artificially injured liver to the infection and development of the liver fluke, Clonorchis sinensis.
Liver cirrhosis, as a liver injury, was attained by administration of carbon tetra-chloride which was administered to rats by means of inhalation, 1 ml. of CC14 being vaporized in 10 liters of air, for a period of ten minutes daily for 28 days. (Berryman and Bollman, 1943)
Experimental groups were divided as following 3 groups, and worm recovery rate, size and morphological changes of the recovered worms were observed on 10, 20, 30, 40, 60 and SO days after infection in each group.
Group 1 : Sixty metacercariae were administered to each rat after 28th day of carbon tetrachloride inhalation in order to evaluate the effect of preceeding liver injury to Clonorchis sinensis.
Group 2: Sixty metacercariae were administered to each rat at 7th day of carbon tetra-chloride inhalation and the inhalation was continued for 21 days in order to observe the status of C. sinensis in the process of liver injury.
Group 3 : Control group. Sixty metacercariae were administered to each rat without liver injury.
The following results were obtained in this study.
1. The worm recovery rates of Group 1 (47.6%) and Group 2 (21.8%) were lower than that of Group 3 (64.2%).
2. The size of worm recovered from Group 1 and Group 2 were smaller than that of Group 3 until the 40th day of infection. Retardation of growth in Group 1 was more
remarkable than Group 2 until 30th day, but there was no significant difference in size of the worms after 40th day of infection.
3. Morphological alterations of recovered worms were not significant in microscopical examination, except scantiness of eggs in uterus and retardation of development of testis in both experimental groups until 40th day of infection.
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